永丰草地的类型及其生产力评估

本文描述了甘肃省天祝县永丰草地的主要草地类型、生产能力及利用模式。研究工作显示,草地改良措施,对不同植被的生物量分布,将产生显著的效果。灌溉和施肥,将促进河谷草甸的地下生物量向地上生物量的转化过程。灌溉可使河谷草甸中禾草组分的增加超过50％,使产草量增加50％～100％。上年秋天和当年春天土壤的含水量,是全年产草量的决定因素,其对产草量的重要性超过全年平均降水量的影响。除了植被组分、产草量的变化外,河谷草地在可消化粗蛋白、可消化能、代谢能等方面也有重大变化,9月份与4月份相比,这三项指标分别高出376％、86％和86％。不同年龄组的绵羊饲料消耗显示,7岁羊的饲料消耗是10月龄羊的17倍;但是7岁羊的胴体重量仅是10月龄羊的2倍。根据永丰滩草地、饲草、家畜的这些特点,当地的畜牧业,不应当在毫无经济意义的情况下,保持太多的牲畜头数,优化畜产品生产的最好方法,就是实行季节畜牧业。季节畜牧业(其中繁殖母羊数达67％)与其他草地改良措施相结合,将使该地区单位面积上的绵羊的产肉量提高4.9倍。     

中国野生种毛葡萄光合特性的研究

毛葡萄（ＶｉｔｉｓｑｕｉｎｑｕａｎｇｕｌａｒｉｓＲｅｈｄ）的净光合速率日变化值明显高于山葡萄、宝石、白玉霓，且无栽培品种光合作用的“午休”现象。毛葡萄的净光合速率与其它野生种葡萄和栽培品种比较也表现出较高水平。在进行ＣＯ２饱和点和补偿点测定时，毛葡萄也较栽培品种白玉霓有较强的光合作用，因此，毛葡萄是葡萄高光合效率育种的优良野生种质资源。     

Sensitivity of four Yeasts to Fungicides and CO2 Concentrations and Their Antagonistic Ability in Combination with Fungicide to pathogenic Fungi in vitro

The yeasts Cryptococcus laurentii,Trichosporon pullulans,Rhodotorula glutinis and Trichosporon sp.were used to investigate their sensitivity to four fungicides and different concentrations of CO2,as well as their antagonistic ability to Penicilliumexpansum and Alternaria alternata in vitro when applied with fungicide.There were significant differences in sensitivity to the fungicides among the different yeasts(P = 0.05).R.glutinis was more sensitive to Deccocil,Iprodione and Stroby as compared to other yeasts.Combination antagonistic yeasts with fungicide could more significantly enhance biocontrol ability of the yeasts against the pathogenic fungi in vitro(P = 0.05).C.laurentii was the most effective antagonist among the four yeasts and could completely control spore germination of P.expansum and A.alternata when combined with Stroby at the concentration of 100 μL L-1.The yeasts,except R.glutinis,could grow well on nutrient yeast dextrose agar (NYDA) after 8 d incubation even at 20% CO2 concentration at 25℃.Particularly Trichosporon sp.showeda better adaptability to low temperature as compared to other antagonists.     

一条不对称合成S(+)-2-氨基-4-磷酸基丁酸的新路线

利用4-二乙氧基膦酰基-2-酮丁酸乙酯与 L-赤式-(+)-1,乙-二苯基-2-氨基乙醇的缩合,将产物还原、氢解及水解得光活性产物,ee 值67%。     

白细胞介素和干扰素γ对绵羊体外受精胚胎体外发育影响的研究

利用SOFM+0.8%BSA+1mM谷氨酰胺作为绵羊胚胎的培养液,分别添加LIF、IL-1β、IL-2、IL-6及IFN-γ,以研究LIF、IL-1β、IL-2、IL-6及IFN-γ对绵羊早期胚胎发育的影响。结果说明,LIF促进2～8细胞胚胎发育至桑椹胚,同时也能够促进桑椹胚向扩大囊胚和孵化囊胚发育。但IL-1β、IL-2、IL-6及IFN-γ对绵羊早期胚胎的发育无显著影响。     

Analysis on Mineral Element Contents in Associated with Varietal Type in Core Collection of Yunnan Rice

Eight-element contents of 653 unpolished rice samples harvested from Xingping experiment farm, Yunnan Province under the same ecological conditions were analyzed by ICP-AES method. The mineral elements content were closely related to low diversity, high-yielding, and multi-resistance breeding; The K, Mg, Ca and Mn content in high-yielding and resistant varieties were high, and other nutrients such as P, Fe, Zn and Cu were low, which was connected with the heredity and physiological mechanism of mineral nutrients. There is zonal distribution of mineral elements content from Yunnan rice, especially for P, Fe, Zn and Cu co-related with the diversity center, paddy versus upland, glutinous and non-glutinous, glume-hair versus nuda, rice color, rice flavor, soft rice versus non-soft rice, but it did not find any association with indica-japonica types. The results supported the ecological variety group view of 5-grade taxonomic system “species-subspecies-ecological groups-ecological variety groups - varietal types“ .     

蜂胶对糖尿病大鼠血糖的影响

将复制糖尿病模型的 72只SD大鼠随机分为模型组 ,蜂胶水提液低剂量组、高剂量组 ,蜂胶醇提液低剂量组、高剂量组和阳性对照组 (拜糖苹 ) ,另取 12只为正常组。除正常组和模型组外 ,各组分别给予不同的试验药物。给药过程中 ,每周取血一次 ,测空腹血糖 ,给药 7周后 ,取血测定空腹血糖和果糖胺。试验结果表明 ,蜂胶水提液和醇提液均能控制糖尿病大鼠空腹血糖的升高 ,并且随着时间的延长效果越来越明显。     

猪肺炎支原体黏附因子基因R1R2区的克隆及表达

根据GenBank登录的猪肺炎支原体232株P97基因和J株黏附因子基因设计了1对引物，以我国猪肺炎支原体Z株(强毒株)基因组DNA为模板，通过PCR方法扩增了该株黏附因子基因的部分序列。经序列分析后，重新设计了1对带有EcoRI和HindⅢ酶切位点的引物，并经引物的定点突变，PCR扩增了Z株黏附因子的R1R2区。扩增产物经双酶切后克隆到表达载体pET-32(a) 中。该重组质粒经酶切鉴定后，将其具有正确阅读框架的重组质粒转化到大肠杆菌BL21(DE3)感受态细胞，37℃下经IPTG诱导表达，得到相对分子质量约29000的融合蛋白，表达量约为11％。     

超临界CO_2流体处理工艺对木材渗透性的影响

以苯—乙醇为夹带剂,研究超临界CO2流体不同处理压力、温度、时间对福建产杉木、马尾松、檫木木材渗透性的影响。结果表明,在35 MPa内,处理压力的增大有利于3种木材渗透性的提高;杉木在处理温度为40℃时,渗透性的变化率达到最大,为493%,马尾松和檫木在处理温度为45℃时,渗透性的变化率达到最大,分别为479%和349%;较佳的处理时间为30 min。